| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.096924 |
| Chromosome: | chromosome 6 |
| Location: | 8446567 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307300 | ANT2 | ADP/ATP carrier protein, mitochondrial; (1 of 3) K05863 - solute carrier family 25 (mitochondrial adenine nucleotide translocator), member 4/5/6/31 (SLC25A4S, ANT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGATGCATTTGGGCGTCCCGGTGCAT |
| Internal bar code: | CTTACATTCGTCTTCCTTTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 98.7912 |
| LEAP-Seq n confirming: | 2370 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGACCCACTCCGAGTTC |
| Suggested primer 2: | GGCCATTGCGTTTACTTGAT |