| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.096931 |
| Chromosome: | chromosome 12 |
| Location: | 8269425 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550200 | SMM44 | (1 of 2) 2.1.1.9 - Thiol S-methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGCAAATTAATGCGAGCGACAGTAGTA |
| Internal bar code: | CCTGGGAGAGTCTATACAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 79.4319 |
| LEAP-Seq n confirming: | 2321 |
| LEAP-Seq n nonconfirming: | 601 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACAGAACCTGAGAGGTC |
| Suggested primer 2: | CTACCTGACAGCTGTGCCAA |