| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096948 |
| Chromosome: | chromosome 13 |
| Location: | 605471 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565675 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGCGGTCCGGTGGCTGTAGTGCATGGGA |
| Internal bar code: | GACTAGGCATAAAGAGTGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 92.734 |
| LEAP-Seq n confirming: | 8551 |
| LEAP-Seq n nonconfirming: | 670 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGTCCTACCACCAGCACC |
| Suggested primer 2: | GGGAATATCGCGACTGATGT |