| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096966 |
| Chromosome: | chromosome 9 |
| Location: | 3174443 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387245 | STT3A | Staurosporin and temperature sensitive 3-like A; (1 of 1) PTHR13872//PTHR13872:SF1 - 60S RIBOSOMAL PROTEIN L35 // DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGCTGTTGAGCATGGTCTTCGCGCCGG |
| Internal bar code: | CGAACGTGCCTGAAAGGTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 61.0626 |
| LEAP-Seq n confirming: | 3850 |
| LEAP-Seq n nonconfirming: | 2455 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTCGCAACTCTCCATC |
| Suggested primer 2: | GGATGTTTGGAAAAGCGGTA |