| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096985 |
| Chromosome: | chromosome 1 |
| Location: | 2992082 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACATTATTTATCATGCCTTTGTCCAAGA |
| Internal bar code: | GCTATGCACTGTAGGGAGCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 97.5484 |
| LEAP-Seq n confirming: | 3223 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGCCAGTCTAATGGTGAC |
| Suggested primer 2: | GTACTGCCACCGAACTCCAT |