Insertion junction: LMJ.RY0402.096994_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.096994
Chromosome:chromosome 14
Location:213600
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g609100 (1 of 6) PTHR13339//PTHR13339:SF0 - COP9 SIGNALOSOME COMPLEX SUBUNIT 8 // COP9 SIGNALOSOME COMPLEX SUBUNIT 8 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCAACAACATGCACGCCATCCACACCAC
Internal bar code:GCGTCGTCCAAAGGGCGCTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:736
LEAP-Seq percent confirming:95.2174
LEAP-Seq n confirming:3942
LEAP-Seq n nonconfirming:198
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCCTGTCAAGTTGGTAAA
Suggested primer 2:CACGTCCAGAACCTCCAAAT