| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097005 |
| Chromosome: | chromosome 6 |
| Location: | 5285913 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282800 | ICL1 | Isocitrate lyase; (1 of 1) 4.1.3.1 - Isocitrate lyase / Isocitritase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAGCCTGCTCCCCCTCCTCCCCCCCCCC |
| Internal bar code: | GGGACACGCCAGTCTCGACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATACGAGGTGAGTGAGCA |
| Suggested primer 2: | ATACAAGGGCCTCCTCTGGT |