Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.097016 |
Chromosome: | chromosome_6 |
Location: | 6509155 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g293150 | PGM12 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCGCCAGAGGCCCTTTTTGGCAAATTTCG |
Internal bar code: | CAAAAGGCGTTAACATTGCAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 649 |
LEAP-Seq percent confirming: | 98.8117 |
LEAP-Seq n confirming: | 2744 |
LEAP-Seq n nonconfirming: | 33 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCACATGATTTGTTTGTGC |
Suggested primer 2: | AGTGGCGACTTTTAGCCTGA |