Insertion junction: LMJ.RY0402.097019_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.097019
Chromosome:chromosome 11
Location:3058496
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g478950 (1 of 1) K14567 - U3 small nucleolar RNA-associated protein 14 (UTP14) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTGTGTTTGTAGACTGTGCCTGCCAGAG
Internal bar code:TGGTTACATATATGACGGGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2115
LEAP-Seq percent confirming:99.9231
LEAP-Seq n confirming:5198
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGTTGTTTTACGGGCTGT
Suggested primer 2:TTGGTGTTGTGAGCATTGGT