| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097019 |
| Chromosome: | chromosome 16 |
| Location: | 2352013 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659700 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCCTCCGCCAGCGGCTTCCCCATCTG |
| Internal bar code: | GGCCCCGAATCCGTTGGACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 98.8489 |
| LEAP-Seq n confirming: | 2748 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCTCCTTGAGTGGTTGC |
| Suggested primer 2: | GGACCTGAGCAACATCCAGT |