Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.097026 |
Chromosome: | chromosome_1 |
Location: | 5006279 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g034550 | FAP109 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTGCCGAAGCAATATGTGACGTTGACTAC |
Internal bar code: | CGGGCCGAACCCGCGCGGCGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 623 |
LEAP-Seq percent confirming: | 99.3782 |
LEAP-Seq n confirming: | 3516 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCTTGTCGAGTTGGTGCTT |
Suggested primer 2: | GTTAGCTTAGGCGCGACAAC |