| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097042 |
| Chromosome: | chromosome 12 |
| Location: | 2874123 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502179 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCAGCCCATGACAGCAGCAGCGCTGCC |
| Internal bar code: | TTTACGGCACTGGTGCGGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 400 |
| LEAP-Seq percent confirming: | 95.2409 |
| LEAP-Seq n confirming: | 3202 |
| LEAP-Seq n nonconfirming: | 160 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAATAATGCGCCTGCTGCT |
| Suggested primer 2: | AAGATTGTTGGCAGTTTGGG |