Insertion junction: LMJ.RY0402.097071_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.097071
Chromosome:chromosome_6
Location:2171429
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g265950 DHC3 Flagellar inner arm dynein heavy chain, novel minor type dynein sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TAACACCACACCTGCTGTCTACTTCGCTAC
Internal bar code:CTGGGCGCTGTCCTGTGTGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:132
LEAP-Seq percent confirming:99.4505
LEAP-Seq n confirming:181
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAACAGTACCATAGCCGC
Suggested primer 2:CCGTGGCATAGCACATACAC