| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097082 |
| Chromosome: | chromosome 7 |
| Location: | 3637474 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337050 | (1 of 1) K13346 - peroxin-10 (PEX10) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATAGTAAAATATACTGCGCTAATTAGCGT |
| Internal bar code: | TGACGAGCGACTGGTTGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 777 |
| LEAP-Seq percent confirming: | 98.9478 |
| LEAP-Seq n confirming: | 2539 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGAAACATGAACGAAAGG |
| Suggested primer 2: | AGTCGGTCATAGTGCAAGCC |