| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097092 |
| Chromosome: | chromosome 8 |
| Location: | 885226 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361350 | (1 of 3) PF01936 - NYN domain (NYN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATACCTACTCACTTCACGAGACACACACA |
| Internal bar code: | CGCTGGACGGTCCGGCTGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6 |
| LEAP-Seq percent confirming: | 50.6019 |
| LEAP-Seq n confirming: | 1093 |
| LEAP-Seq n nonconfirming: | 1067 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCTGTTGTTGCTGTGGT |
| Suggested primer 2: | ATGCCCTATCTGTCGTCCAC |