| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.097111 |
| Chromosome: | chromosome 6 |
| Location: | 6080085 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g289750 | CYCD2 | D-type cyclin; (1 of 3) K06627 - cyclin A (CCNA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATTTGAACCCGTTAACTCCACCCGGTT |
| Internal bar code: | TCGAAGGTGATTGGTAGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTGAAGAGGGGTGGTGT |
| Suggested primer 2: | TATAAGCCCTGCATCCAACC |