| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097133 |
| Chromosome: | chromosome 9 |
| Location: | 3299923 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388208 | (1 of 1) 1.13.11.24 - Quercetin 2,3-dioxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGATGACAAGACCAAGGTAAGGGCTTGA |
| Internal bar code: | GTACCAGTGTGGGGCCCCCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 948 |
| LEAP-Seq percent confirming: | 99.4732 |
| LEAP-Seq n confirming: | 14350 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCTCACGCGTAACATGC |
| Suggested primer 2: | CATCTCCACCACCAGGAAGT |