| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097138 |
| Chromosome: | chromosome 14 |
| Location: | 2010336 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621501 | CDS|outside_mRNA | ||
| Cre14.g621600 | (1 of 1) PF15003 - HAUS augmin-like complex subunit 2 (HAUS2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCAAGGGCGCGGCGGCGACAGGGCCGT |
| Internal bar code: | TAACAAGCGACCGTCGAGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACAGCAGCCTGTGGAG |
| Suggested primer 2: | CTAAGCAAGCTGATGGAGGC |