Insertion junction: LMJ.RY0402.097144_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.097144
Chromosome:chromosome 5
Location:2943255
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre05.g238260 (1 of 1) PF13371//PF13414//PF13432 - Tetratricopeptide repeat (TPR_9) // TPR repeat (TPR_11) // Tetratricopeptide repeat (TPR_16) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTTTGGGAGGCGGGAAAGGGGCGGAGGG
Internal bar code:GGGCGAAAGCCTGGGGGTTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:221
LEAP-Seq percent confirming:29.9602
LEAP-Seq n confirming:752
LEAP-Seq n nonconfirming:1758
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCCTGTCCCTGTCCCT
Suggested primer 2:CCATTCTTGTGAACGGTGTG