| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097155 |
| Chromosome: | chromosome 1 |
| Location: | 4142384 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027350 | TMG10 | (1 of 2) PTHR12029:SF11 - METHYLTRANSFERASE TARBP1-RELATED; tRNA (guanosine-2'-O)-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGAGGGGAGGCCGCTTCGGCGGCCGAT |
| Internal bar code: | AGTATCGTTCGGTCTGGGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 905 |
| LEAP-Seq percent confirming: | 99.4436 |
| LEAP-Seq n confirming: | 1966 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGTGTGTGAGAGCATCG |
| Suggested primer 2: | GCTGTACGACTATGTGCGGA |