| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097160 |
| Chromosome: | chromosome 12 |
| Location: | 6087768 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535700 | HSP70D | (1 of 2) PTHR19375:SF173 - CHAPERONE PROTEIN DNAK; Heat shock protein 70D | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAGGGGAGAGGATGGGGTGCGCACACT |
| Internal bar code: | ATGATGCAGCGACCTTTTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 99.7459 |
| LEAP-Seq n confirming: | 1963 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGAGTCCTACAACAGCC |
| Suggested primer 2: | TGGCTACGCCTTCACTTCTT |