| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.097187 |
| Chromosome: | chromosome 17 |
| Location: | 2856634 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719325 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCGTGCCTCTGACTGAGCACCTAGCG |
| Internal bar code: | ATACCATCTGCTACTGAGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 135 |
| LEAP-Seq percent confirming: | 97.3199 |
| LEAP-Seq n confirming: | 1162 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTACCGAGTCAGAACTGC |
| Suggested primer 2: | TGATCTGTTTGTCTGCCAGC |