Insertion junction: LMJ.RY0402.097190_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.097190
Chromosome:chromosome 2
Location:3940133
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g096551 (1 of 230) IPR017986 - WD40-repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGTCGTTGTGCTGAACATATCATATGTG
Internal bar code:TACGTTGGGGCGTGAACTCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:463
LEAP-Seq percent confirming:97.504
LEAP-Seq n confirming:2422
LEAP-Seq n nonconfirming:62
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGAAGGTTGTGAGTGTGT
Suggested primer 2:ACACACACACACACACACGC