| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097204 |
| Chromosome: | chromosome 14 |
| Location: | 3976943 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633300 | (1 of 1) IPR003582//IPR006626//IPR009030 - ShKT domain // Parallel beta-helix repeat // Insulin-like growth factor binding protein, N-terminal | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGAGGGAGTTGGGTTGCGGGAGCAGGC |
| Internal bar code: | GGATCGGTCAATCACAGGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 89.2064 |
| LEAP-Seq n confirming: | 281 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGCTTCATCACTGTGTC |
| Suggested primer 2: | AGGACAGCATATTCGGCAGT |