| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.097205 |
| Chromosome: | chromosome 16 |
| Location: | 3732410 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689050 | ACX1,ACO1 | Acyl-CoA oxidase/dehydrogenase; (1 of 1) PTHR10909//PTHR10909:SF250 - ELECTRON TRANSPORT OXIDOREDUCTASE // PEROXISOMAL ACYL-COENZYME A OXIDASE 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAGCGGGCCCCAGTGACAGGGGAAGGT |
| Internal bar code: | AGTCTGCGACCGTCTGCATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 272 |
| LEAP-Seq percent confirming: | 99.1784 |
| LEAP-Seq n confirming: | 845 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGTGTGTGTAGAGGGG |
| Suggested primer 2: | GAGGGCGGCTTTCATACATA |