| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.097214 |
| Chromosome: | chromosome 10 |
| Location: | 6507286 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466550 | TF2H1 | General transcription factor IIH subunit 1; (1 of 1) IPR005607//IPR027079 - BSD domain // TFIIH subunit Tfb1/p62 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCTCGCCCTCAGTCCGGCCAGTTCAGA |
| Internal bar code: | CAGGGTACGCACACTGGTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 461 |
| LEAP-Seq percent confirming: | 94.8732 |
| LEAP-Seq n confirming: | 8716 |
| LEAP-Seq n nonconfirming: | 471 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTAATCGATCAGCTTGCG |
| Suggested primer 2: | CAGCCTGTCTCTCTTGACCC |