| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097221 |
| Chromosome: | chromosome 7 |
| Location: | 4193525 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342250 | PFD2 | Prefoldin molecular chaperone, subunit 2; (1 of 1) K09549 - prefoldin subunit 2 (PFDN2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGCTTATTATGCAGGCGTGGCGGGAGA |
| Internal bar code: | TCCACGCGCGGGAAAGGGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 99.0196 |
| LEAP-Seq n confirming: | 2323 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGTAGACACGGCATGAG |
| Suggested primer 2: | CTCAACCTGTCAACCCCACT |