Insertion junction: LMJ.RY0402.097232_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.097232
Chromosome:chromosome 10
Location:2335664
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g434850 (1 of 14) IPR006626//IPR011050 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATTGCAAACGGGCAGCCCCCACCTCCAAC
Internal bar code:GTTCGATGATGGAAGTGGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:319
LEAP-Seq percent confirming:85.9504
LEAP-Seq n confirming:104
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCCTTTGGATTCACCGTC
Suggested primer 2:TAGGTGTACGTGTGCGCTTC