Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.097232 |
Chromosome: | chromosome 10 |
Location: | 2335664 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g434850 | (1 of 14) IPR006626//IPR011050 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGCAAACGGGCAGCCCCCACCTCCAAC |
Internal bar code: | GTTCGATGATGGAAGTGGTGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 319 |
LEAP-Seq percent confirming: | 85.9504 |
LEAP-Seq n confirming: | 104 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTCCTTTGGATTCACCGTC |
Suggested primer 2: | TAGGTGTACGTGTGCGCTTC |