Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.097248 |
Chromosome: | chromosome 16 |
Location: | 6032675 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g676350 | (1 of 1) PTHR23073//PTHR23073:SF37 - 26S PROTEASE REGULATORY SUBUNIT // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGCTTCAAAGCTCAACACGCCCCGCCC |
Internal bar code: | GCGCGAGGCCTTCGTACGGTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 185 |
LEAP-Seq percent confirming: | 99.1518 |
LEAP-Seq n confirming: | 12040 |
LEAP-Seq n nonconfirming: | 103 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGGAACGTTTGCCTAATGC |
Suggested primer 2: | GTTGTGACCTGATGTGGACG |