| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097250 |
| Chromosome: | chromosome_8 |
| Location: | 1034405 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g362050 | FAP49 | Flagellar Associated Protein, membrane-associated | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCCCGAGCCCACATGGCGCGACTGGGAAT |
| Internal bar code: | CGGACGTAGCTGAGGTGCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 98.2147 |
| LEAP-Seq n confirming: | 7757 |
| LEAP-Seq n nonconfirming: | 141 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGATCCAGCCCTATCACTA |
| Suggested primer 2: | CTGGAAGGCAGCAAGTAAGG |