| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097252 |
| Chromosome: | chromosome 14 |
| Location: | 3099324 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628650 | MME4 | NADP-dependent malic enzyme 4; (1 of 5) 1.1.1.40 - Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) / Pyruvic-malic carboxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGTATTGGGCTCGGCGGGCACATCACCC |
| Internal bar code: | ACTGGTGCACTGTACCGTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 97.5155 |
| LEAP-Seq n confirming: | 157 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGCCTTACTTCCTAGCCA |
| Suggested primer 2: | TAATGTGTGGGAGCGGTACA |