| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097259 |
| Chromosome: | chromosome 2 |
| Location: | 7578544 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143801 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCGCCAAGAAGCGCATTAGTCGTTGAG |
| Internal bar code: | TTGTTCGCGGTTACGTCCTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 951 |
| LEAP-Seq percent confirming: | 99.7058 |
| LEAP-Seq n confirming: | 3389 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTACACATGCTTCCACAC |
| Suggested primer 2: | AGAAAGCGGGAAGACTGTGA |