| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.097263 |
| Chromosome: | chromosome 1 |
| Location: | 4448272 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030200 | PP2C4,PPP3,FAP6 | Flagellar Associated Protein 6; (1 of 1) PTHR13832:SF273 - PROTEIN TAP-1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAAGCCTGTGCGTGTCCGTGACTCCGTC |
| Internal bar code: | TTCTCAGACCTAGGGCACGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 408 |
| LEAP-Seq percent confirming: | 98.8658 |
| LEAP-Seq n confirming: | 523 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCGTAGCTGCATGGACA |
| Suggested primer 2: | AGGTCCACCTTCACCTCCTT |