Insertion junction: LMJ.RY0402.097263_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.097263
Chromosome:chromosome_1
Location:4448272
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre01.g030200 PP2C4,PPP3 Phosphoprotein phosphatase 2C delta sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GCAAAGCCTGTGCGTGTCCGTGACTCCGTC
Internal bar code:TTCTCAGACCTAGGGCACGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:408
LEAP-Seq percent confirming:98.8658
LEAP-Seq n confirming:523
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCGTAGCTGCATGGACA
Suggested primer 2:AGGTCCACCTTCACCTCCTT