Insertion junction: LMJ.RY0402.097269_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.097269
Chromosome:chromosome_6
Location:2166311
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g265950 DHC3 Flagellar inner arm dynein heavy chain, novel minor type dynein sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TCGCCGCTGTCGCTGTACGACGTGCACCCC
Internal bar code:ATCCCGATTTTGCCCCCACGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:472
LEAP-Seq percent confirming:39.8524
LEAP-Seq n confirming:7990
LEAP-Seq n nonconfirming:12059
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCTGTTCACCGACTCCTC
Suggested primer 2:ACCCTCCACCAGGAACATCT