| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097271 |
| Chromosome: | chromosome 3 |
| Location: | 4941947 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179921 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGGCGCATGCACCGACTTCTGAAGTTG |
| Internal bar code: | TAGACCTTAAGCGAAATTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 807 |
| LEAP-Seq percent confirming: | 91.0212 |
| LEAP-Seq n confirming: | 6346 |
| LEAP-Seq n nonconfirming: | 626 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCCACTTATGTACGGCA |
| Suggested primer 2: | GGCTCGTAATTCCCTAAGGC |