| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.097272 |
| Chromosome: | chromosome 6 |
| Location: | 1639970 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260850 | NOP14 | (1 of 1) K14766 - nucleolar protein 14 (NOP14, UTP2); Nucleolar protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCATTGTTGCCAGTTCGCATTCATTTGG |
| Internal bar code: | GTTTGGTTGTATGACGATCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 641 |
| LEAP-Seq percent confirming: | 99.6938 |
| LEAP-Seq n confirming: | 6187 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTGTTCATAGAGCCGTG |
| Suggested primer 2: | GCTTCGAGGAGGACTACGTG |