Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.097296 |
Chromosome: | chromosome 9 |
Location: | 5843489 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g402256 | (1 of 3) 3.4.11.1 - Leucyl aminopeptidase / Peptidase S | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCATGATTAGTCAAGAAGTGAAGGCGTA |
Internal bar code: | ATAGCTTTGCATCGCCGCGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 533 |
LEAP-Seq percent confirming: | 99.2259 |
LEAP-Seq n confirming: | 5127 |
LEAP-Seq n nonconfirming: | 40 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GATGAAGGCCTGTGTGTGTG |
Suggested primer 2: | TACCTTGTGTGGTTTTGCCA |