Insertion junction: LMJ.RY0402.097305_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.097305
Chromosome:chromosome 2
Location:6241685
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g114150 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGTTTATGCCACCGTCGCACTCGCACTT
Internal bar code:GGAACAGCGGATGCTGGGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:119
LEAP-Seq percent confirming:99.1936
LEAP-Seq n confirming:123
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CATTCTTTGTGGAGCAGCAA
Suggested primer 2:CGTTCGTGGTATACCGCTTT