| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097307 |
| Chromosome: | chromosome 4 |
| Location: | 3050585 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225200 | FAP311 | Flagellar Associated Protein 311; (1 of 5) PF13374 - Tetratricopeptide repeat (TPR_10) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGCTATTGAGAGCACAACGGCCCTGGT |
| Internal bar code: | CGACAGCCATGCCGGCGTAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 13 |
| LEAP-Seq percent confirming: | 97.7273 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCTCAAAGGAACAGGTC |
| Suggested primer 2: | TCCCATCTCTGACCTCATCC |