Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.097316 |
Chromosome: | chromosome 9 |
Location: | 7206922 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGCTGCGGTGCTCCGTGCATCCATTGA |
Internal bar code: | GGTCAAGACGGCTTGGCTTTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 685 |
LEAP-Seq percent confirming: | 99.2783 |
LEAP-Seq n confirming: | 2476 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGTTGTAAGGGAGGGGTGG |
Suggested primer 2: | ACCTCCACCACTGAGTCCAC |