| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.097323 |
| Chromosome: | chromosome 17 |
| Location: | 921405 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g702850 | CAV5 | (1 of 5) PTHR10037//PTHR10037:SF218 - VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM // SUBFAMILY NOT NAMED; Voltage-gated Ca2+ channel, alpha subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGGATCGGGTGATGGCGCTGCTGGGTG |
| Internal bar code: | CCGGCACGCTCCGATGGGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 96 |
| LEAP-Seq percent confirming: | 33.4158 |
| LEAP-Seq n confirming: | 135 |
| LEAP-Seq n nonconfirming: | 269 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCAGTACAACCCAGTGTA |
| Suggested primer 2: | GAAGACGGCTGCGACTGT |