| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097330 |
| Chromosome: | chromosome_17 |
| Location: | 3218469 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g722350 | RABL2A | Small Rab-related GTPase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTAGGGCTGAGCGGTGGTTCGGGGAAAAC |
| Internal bar code: | TATGGCCGCGAGAGCTCGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 117 |
| LEAP-Seq percent confirming: | 95.0893 |
| LEAP-Seq n confirming: | 213 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTCCAAACTCACAGTCC |
| Suggested primer 2: | ACGTCATAGACCAACCCTCG |