| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097344 |
| Chromosome: | chromosome 1 |
| Location: | 5622690 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039950 | MFT32 | Major facilitator superfamily transporter; (1 of 1) PF06813 - Nodulin-like (Nodulin-like) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCGTAAAGATGGAACATGCGTAAGCGA |
| Internal bar code: | GTGTCAGTGGCGTTCAAAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 98.4911 |
| LEAP-Seq n confirming: | 718 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGAGACCACATGCACAC |
| Suggested primer 2: | GGTCATTCTTGTTGGGCTGT |