| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097368 |
| Chromosome: | chromosome 1 |
| Location: | 5718927 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040801 | (1 of 3) PTHR18937//PTHR18937:SF254 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACAGCCCCGACCCCCCAGCAGCGGCAC |
| Internal bar code: | CCCGGACGAGGTAGTGTAAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 282 |
| LEAP-Seq percent confirming: | 99.0244 |
| LEAP-Seq n confirming: | 203 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGCTGGTTAGCGATTTC |
| Suggested primer 2: | GCCCCTGTCAAGTTGGTAAA |