Insertion junction: LMJ.RY0402.097368_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.097368
Chromosome:chromosome 1
Location:5718928
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g040801 (1 of 3) PTHR18937//PTHR18937:SF254 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAAGTTGGACGGTTGGACGTCGCGACGT
Internal bar code:GCACGAAACGCCGTGAGGACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:494
LEAP-Seq percent confirming:97.2308
LEAP-Seq n confirming:948
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTGCTGGTTAGCGATTTC
Suggested primer 2:GCCCCTGTCAAGTTGGTAAA