Insertion junction: LMJ.RY0402.097387_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.097387
Chromosome:chromosome 15
Location:326841
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre15.g635750 (1 of 1) IPR003613//IPR016024 - U box domain // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGAACTACCGGTACCTCCCCCCAAAGGCC
Internal bar code:TATTCAGTGGGCACTCAACTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:914
LEAP-Seq percent confirming:99.6706
LEAP-Seq n confirming:4842
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGGATTGGATTCAAATGTGG
Suggested primer 2:AGCACACAAGCACACACACA