| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097387 |
| Chromosome: | chromosome 17 |
| Location: | 6079645 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741300 | SAK1 | Regulator of acclimation to singlet oxygen | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTGAATGTGCATATTTAGTTTGTCGTC |
| Internal bar code: | TGCGACGGGGGATCGCGTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 596 |
| LEAP-Seq percent confirming: | 99.2679 |
| LEAP-Seq n confirming: | 1356 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGCATTTAGCCTCCTCT |
| Suggested primer 2: | GGTTCGCTAGCGCTCATAAC |