Insertion junction: LMJ.RY0402.097389_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.097389
Chromosome:chromosome 7
Location:6030833
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g354850 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCTTCAACCCCTCTTCAACCCCCTTTC
Internal bar code:GCGCACACCAGAGTGTGGAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:182
LEAP-Seq percent confirming:98.5795
LEAP-Seq n confirming:347
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGAAGTACAAGAGCCCC
Suggested primer 2:CTGCTGTCACTGCCACTCTC