| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.097391 |
| Chromosome: | chromosome 10 |
| Location: | 41156 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g417750 | (1 of 10) 3.1.1.5 - Lysophospholipase / Phospholipase B | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCAACCGGCTCACATGGTCATTCTGGGC |
| Internal bar code: | CGTTTACTGTTGTATAGCCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 69 |
| LEAP-Seq percent confirming: | 99.5238 |
| LEAP-Seq n confirming: | 627 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCCTATCTAGCAGTCGC |
| Suggested primer 2: | CCAACAAATTGTGCGATGTC |