| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097410 |
| Chromosome: | chromosome 6 |
| Location: | 5031558 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280300 | (1 of 1) PTHR11254//PTHR11254:SF324 - HECT DOMAIN UBIQUITIN-PROTEIN LIGASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGACCTACCGCCTCGTCCCCCAAAGGT |
| Internal bar code: | ATTCAGAAAGGCGGGAGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 838 |
| LEAP-Seq percent confirming: | 98.9137 |
| LEAP-Seq n confirming: | 1639 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTATGGTACCGTGCAGGC |
| Suggested primer 2: | GGTCCGACATGGCTTACAGT |